（１． 中國科學(xué)院大學(xué)生命科學(xué)學(xué)院，北京?。保埃埃埃矗梗?br /> （２． 中國科學(xué)院動物研究所生物膜與膜生物工程國家重點實驗室，北京?。保埃埃保埃保?br /> Impact of Skin Allograft Ｒejection on Thymocyte Subsets in Mice
摘要:目的觀察同種異基因小鼠皮膚移植急性排斥對受體小鼠胸腺細(xì)胞亞群組成的影響。方法以BALB / c 小 鼠作為供體，C57BL /6 為受體，建立小鼠同種異基因皮膚移植模型，于術(shù)后第7 天拆線，第10 天取胸腺，制備胸腺單 細(xì)胞懸液，通過流式細(xì)胞儀檢測胸腺細(xì)胞各個亞群的比例。結(jié)果與對照組相比，同種異基因皮膚移植小鼠胸腺 明顯萎縮，胸腺細(xì)胞總數(shù)明顯減少，胸腺細(xì)胞亞群細(xì)胞數(shù)量顯著降低，同時，調(diào)節(jié)性T 細(xì)胞及胸腺細(xì)胞發(fā)育和遷出 不同階段細(xì)胞絕對數(shù)均減少。結(jié)論皮膚移植排斥反應(yīng)明顯抑制受體小鼠胸腺及胸腺細(xì)胞的發(fā)育。該研究結(jié)果 提示，急性移植排斥反應(yīng)對中樞免疫器官胸腺中的T 細(xì)胞發(fā)育分化具有重要影響。
關(guān)鍵詞： 同種異基因皮膚移植 小鼠 排斥反應(yīng) 胸腺細(xì)胞
Abstract： Abstract:Objective To investigate the impacts of allograft rejection on the development of thymocyte subsets in a mouse allogeneic skin transplantation model． Method Skin tissues from BALB / c mice were transplanted into C57BL /6 mice． The total cell number of thymocytes and their subsets in allo-skin-grafted recipients were analyzed by flow cytometry． Ｒesult In allo-skin transplantation group，the thymus weight was decreased，the quantities of thymocytes and their subsets including DN，DP，CD4SP and CD8SP cells were significantly decreased compared with the control mice． Conclusion The data showed that the acute rejection significantly impacts the development of T cells in the thymus。
Keywords： skin allograft mice rejection thymocytes
Fund:國家重點基礎(chǔ)研究發(fā)展計劃(973)資助(No． 2010CB945301) ;國家自然科學(xué)基金(No． CB1130055 ) ;中國科學(xué)院創(chuàng)新工程重要方向項目(YSC-X2-YW-Ｒ-238)
引用本文: 張紅梅, 吳亮其, 孫麗娜, 趙勇.異基因皮膚移植對小鼠胸腺細(xì)胞亞群的影響[J] 實驗動物科學(xué), 2014,V31(01): 1-5
Zhang-Hong-Mei, WU Liang-Qi, SUN Li-Na, ZHAO Yong.Impact of Skin Allograft Ｒejection on Thymocyte Subsets in Mice[J] laboratory animal science, 2014,V31(01): 1-5
