維甲酸誘導(dǎo)建立昆明小鼠腭裂模型的實(shí)驗(yàn)研究
Experimental Study on KM Mice-cleft Palate Model Induced by Retinoic Acid
doi：10.3969/j.issn.1006-6179.2014.01.002
摘要： 目的 研究維甲酸(retinoic acid RA)誘導(dǎo)建立昆明小鼠腭裂模型的適宜給藥時(shí)間和劑量.方法 將明確孕期的60只昆明小鼠隨機(jī)分為4組(A、B、C、D),每組15只.在妊娠(gestation day,GD)10 d時(shí)對(duì)A、B、C組小鼠分別管飼維甲酸20 mg/kg、50 mg/kg和80 mg/kg,D組管飼玉米油0.20 mL,所有小鼠均任其妊娠足日生產(chǎn),記錄各組孕鼠死亡率、死胎率及活胎鼠中腭裂發(fā)生率.采用SPSS 16.0軟件包,用x2檢驗(yàn)分析各組間差異.結(jié)果 B組活胎鼠中腭裂發(fā)生率為93.59％,C組活胎鼠中腭裂發(fā)生率為20.00％,二者比較差異有統(tǒng)計(jì)學(xué)意義(P ＜0.007)；C組孕鼠死亡率及死胎率分別為40.00％和76.19％,均明顯高于B組,差異有統(tǒng)計(jì)學(xué)意義(P ＜0.007)；A組與D組中孕鼠未出現(xiàn)死亡,所產(chǎn)胎鼠無(wú)死胎亦無(wú)腭裂發(fā)生.結(jié)論 給予GD 10d昆明小鼠管飼50 mg/kg維甲酸是建立腭裂模型的合適方法.
作者 ： 閆星泉 南欣榮 劉典偉 拜薛鵬
Author： YAN Xing-quan NAN Xin-rong LIU Dian-wei BAI Xue-peng
作者單位 ： 山西醫(yī)科大學(xué)第一醫(yī)院口腔科,太原,030001
刊 名： 實(shí)驗(yàn)動(dòng)物科學(xué) ISTIC
Journal： Laboratory Animal Science
年，卷(期) ： 2014, 31(1)
分類(lèi)號(hào) ： Q95-33
關(guān)鍵詞： 維甲酸 昆明小鼠 腭裂模型
Keywords： RA KM mice cleft palate model
機(jī)標(biāo)分類(lèi)號(hào) ： O65 R78
在線出版日期 ： 2014年5月4日
基金項(xiàng)目 ： 山西省自然科學(xué)基金
Abstract： Abstract:Objective To study when and how much to dose KM mice while creating the cleft palate model induced by ＲA ( retinoic acid) ． Method Divide randomly sixty KM mice which have been identified in pregnancy into four groups (A，B，C，and D)，there were fifteen ones in each group． The mice of group A，B，C were tube-fed ＲA( retinoic acid) with 20 mg / kg、50 mg / kg and 80 mg / kg respectively at the time of GD10( gestation day 10)， while the ones of group D tube-fed with 0. 20 mL corn oil at the same time． All the mice were allowed to reproduce naturally and then record the mortality of pregnant mice，stillbirth rate and incidence of cleft palate in the live fetuses． Data were compared between groups with χ2 test using SPSS16. 0 software package． Ｒesult The incidence of live fetuses with cleft palate in group B was 93. 59%，in group C which was 20. 00%，there was significant difference in the two groups (P ＜ 0. 007)，but the stillbirth rate and mortality of pregnant mice in group C were 40% and 76. 19%，which were higher than those in group B，the differences were statistically significant ( P ＜ 0. 007) ． In addition，the pregnant mice in remaining group A and D were without the occurrence of death and the fetal mice of these groups neither appear stillbirth nor cleft palate deformity． Conclusion Tube-feeding the KM mice with ＲA 50 mg / kg at GD 10 is an appropriate method of setting up cleft palate model
Experimental Study on KM Mice-cleft Palate Model Induced by Retinoic Acid
doi：10.3969/j.issn.1006-6179.2014.01.002
摘要： 目的 研究維甲酸(retinoic acid RA)誘導(dǎo)建立昆明小鼠腭裂模型的適宜給藥時(shí)間和劑量.方法 將明確孕期的60只昆明小鼠隨機(jī)分為4組(A、B、C、D),每組15只.在妊娠(gestation day,GD)10 d時(shí)對(duì)A、B、C組小鼠分別管飼維甲酸20 mg/kg、50 mg/kg和80 mg/kg,D組管飼玉米油0.20 mL,所有小鼠均任其妊娠足日生產(chǎn),記錄各組孕鼠死亡率、死胎率及活胎鼠中腭裂發(fā)生率.采用SPSS 16.0軟件包,用x2檢驗(yàn)分析各組間差異.結(jié)果 B組活胎鼠中腭裂發(fā)生率為93.59％,C組活胎鼠中腭裂發(fā)生率為20.00％,二者比較差異有統(tǒng)計(jì)學(xué)意義(P ＜0.007)；C組孕鼠死亡率及死胎率分別為40.00％和76.19％,均明顯高于B組,差異有統(tǒng)計(jì)學(xué)意義(P ＜0.007)；A組與D組中孕鼠未出現(xiàn)死亡,所產(chǎn)胎鼠無(wú)死胎亦無(wú)腭裂發(fā)生.結(jié)論 給予GD 10d昆明小鼠管飼50 mg/kg維甲酸是建立腭裂模型的合適方法.
作者 ： 閆星泉 南欣榮 劉典偉 拜薛鵬
Author： YAN Xing-quan NAN Xin-rong LIU Dian-wei BAI Xue-peng
作者單位 ： 山西醫(yī)科大學(xué)第一醫(yī)院口腔科,太原,030001
刊 名： 實(shí)驗(yàn)動(dòng)物科學(xué) ISTIC
Journal： Laboratory Animal Science
年，卷(期) ： 2014, 31(1)
分類(lèi)號(hào) ： Q95-33
關(guān)鍵詞： 維甲酸 昆明小鼠 腭裂模型
Keywords： RA KM mice cleft palate model
機(jī)標(biāo)分類(lèi)號(hào) ： O65 R78
在線出版日期 ： 2014年5月4日
基金項(xiàng)目 ： 山西省自然科學(xué)基金
Abstract： Abstract:Objective To study when and how much to dose KM mice while creating the cleft palate model induced by ＲA ( retinoic acid) ． Method Divide randomly sixty KM mice which have been identified in pregnancy into four groups (A，B，C，and D)，there were fifteen ones in each group． The mice of group A，B，C were tube-fed ＲA( retinoic acid) with 20 mg / kg、50 mg / kg and 80 mg / kg respectively at the time of GD10( gestation day 10)， while the ones of group D tube-fed with 0. 20 mL corn oil at the same time． All the mice were allowed to reproduce naturally and then record the mortality of pregnant mice，stillbirth rate and incidence of cleft palate in the live fetuses． Data were compared between groups with χ2 test using SPSS16. 0 software package． Ｒesult The incidence of live fetuses with cleft palate in group B was 93. 59%，in group C which was 20. 00%，there was significant difference in the two groups (P ＜ 0. 007)，but the stillbirth rate and mortality of pregnant mice in group C were 40% and 76. 19%，which were higher than those in group B，the differences were statistically significant ( P ＜ 0. 007) ． In addition，the pregnant mice in remaining group A and D were without the occurrence of death and the fetal mice of these groups neither appear stillbirth nor cleft palate deformity． Conclusion Tube-feeding the KM mice with ＲA 50 mg / kg at GD 10 is an appropriate method of setting up cleft palate model
